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Microbiology Experiments: A Health Science Perspective

Microbiology Experiments: A Health Science Perspective

ISBN 9780077726676
Edition 8
Publication Date
Purchase Type Buy New
Publisher Mcgraw Hill


Basic Microbiology

Introduction to Microbiology 1. Ubiquity of Microorganisms 2. Pure Culture and Aseptic Technique 3. Introduction to the Compound Light Microscope 4. The Oil Immersion Lens

Introduction to Staining of Microorganisms 5. Simple Stains: Positive and Negative Stains 6. Differential and Other Special Stains

Introduction to Microbial Growth 7. Chemically Defined, Complex, Selective, and Differential Media 8. Quantification of Microorganisms

Introduction to the Environment and Microbial Growth 9. Aerobic and Anaerobic Growth 10. The Effect of Incubation Temperature on Generation Time

Introduction to Control of Microbial Growth 11. Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time 12. Control of Microbial Growth with Ultraviolet Light 13. Changes in Osmotic Pressure Due to Salt and Sugar and Their Effects on Microbial Growth 14. Antibiotics 15. Antiseptics and Disinfectants

Introduction to Microbial Genetics 16. Selection of Bacterial Mutants Resistant to Antibiotics 17. Transformation: A Form of Genetic Recombination 18. Gene Regulation: Induction and Catabolite Repression


The Other Microbial World

Introduction to the Other Microbial World l9. Microscopic Identification of Fungi 20. Parasitology: Protozoa and Helminths 21. Titering Prokaryotic Viruses


Microbes and Humans

Introduction to Medical Microbiology 22. Normal Skin Biota 23. Streptococci and Respiratory Microorganisms 24. Identification of Enteric Gram-Negative Rods 25. Clinical Unknown Identification



Introduction to Some Immunological Principles and Techniques 26. Differential White Blood Cell Stains 27. Lysozyme, an Enzyme Contributing to Innate Immunity 28. Enzyme-Linked Immunoabsorbent Assay (ELISA)


Public Health

Introduction to Public Health 29. Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques 30. Microbial Examination of Food 31. Epidemiology


Applications of Biotechnology

Introduction to Biotechnology 32. Identifying DNA with Restriction Enzymes and the Polymerase Chain Reaction (PCR) 33. Electrophoresis 34. Identification of Bacteria Using the Ribosomal Database Project 35. Microarray


Individual Projects

Introduction to the Individual Projects 36. Luminescent Bacteria: Bacteria That Produce Light

APPENDICES 1. Culture Media and Reagent Formulas 2. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in This Manual 3. Dilution Practice Problems 4. Alternative Procedures 5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size 6. History and Working Principles of Light Microscopy

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