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16. The Eadie-Hofstee Equation One transformation of the Michaelis-Menten equation is the Lineweaver-Burk, or double-reciprocal, equation. Multiplying both sides of the Lineweaver Burk equation by Vmax and rearranging gives the Eadie-Hofstee equation:

 

A plot of V0 vs. V0/[S] for an enzyme-catalyzed reaction is shown below. The curve labeled “Slope _ –Km” was obtained in the absence of inhibitor. Which of the other curves (A, B, or C) shows the enzyme activity when a competitive inhibitor is added to the reaction mixture? Hint: See Equation 6–30.

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