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Science · Chemistry
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[40 Marks] Q 4(c) Retention factors for three compounds separated on a nonpolar C8 column were obtained with a methanol/buffer mobile phase at three different pHs, with results shown in Table 2. Explain the elution order and retention data observed Table 2: Retention factors for separation of analytes acetophenone, salicyclic acid and nicotine at pH 3, pH 5 and pH 7. pH 3 4.21 pH5PH7 4.37 Analyte Acetophenone Salicylic acic Nicotine 4.28 2.97.33.11 0.62 0.00 0.13 CHa он CH он salicylic acid pK, 2.97 acetophenone nicoine pkai 3.15 pKa2 7.85

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