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Science · Chemistry
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A chromatography column is typically described by its number of theoretical plates, N, where N is defined by the equation N=16 (tr/w) 2 . The terms tr and σ are defined in the figure. Calculate N for each of the three peaks in the chromatogram and state whether N is constant.

Component A 360 s 8,46 s a 3.6 s Unretained solute 3.52 s 1.5 s 200 100 300 Time (s) Idealized liquid chromatographic separation of three components. 500 Component B t, 600 s 14.10 s 6.0 s 600 700

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