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Science · Chemistry
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PRE-LABORATORY ASSIGNMENT for CHROMATOGRAPHY Hypothetical Chromatogram L Solvent Front line Spotting line solution containing 0A52 goff copper ion per 100 ml to make a spot approximately 0A5 cm in diameter. Calculate the micrograms of copper ion in the spot. Using the ruler in the picture of the chromatogram and measuring with an accuracy of .1 unit, calculate the retardation factor (R) for compound. ....A. MEASUREMENTS ANDCALCULATIONS. units. la) How far did compund A move? units lb) At the same time, how far did the solvent move? lc) What is the Rf for compound A? 2) How many pg (micrograms) of copper ion are in the spot? Why do la. Ib, and 2 have units wheras Ic doesnot? Any help is greatly appreciated!!!
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