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3. How would transcription of the E. coli trp operon be affected by the following manipulations of the leader region of the mRNA? a. Increasing the distance (number of bases) between the leader peptide gene and sequence b. Increasing the distance between sequences 2 and 3 c. Removing sequence 4 e. Eliminating the ribosome-binding site for the gene that encodes the leader peptide Changing several nucleotides in sequence 3 so that it can base-pair with sequence 4 but not with sequence 2
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