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I am usure about #c and have too many answers for #dE. Below, Ive listed a number of either simplifications to DNA replication process or alternative the experimental approaches (like temperature above) allow us to remove the need of an accessory factor or more. For each, list the factors that are no longer needed for DNA replication a. Temperature is temporarily raised to 99 degrees (1 factor) Hellcase b. Addition of two chemically synthesized oligos to use as primers 20 bp long) (1 factor) Primase C. Synthesized oligos are DNA and not RNA based (1 factor) d. Leading and lagging strand is not replicated at the same site (2 factors) single stranded binding proteins Telomerase Ligase

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